DNA & RNA Synthesis

Oligo Synthesis
One of the most important factors in successful automated DNA sequencing is proper primer design.
Primer length and sequence are of critical importance in designing the parameters of a successful amplification: the melting temperature of a NA duplex increases both with its length, and with increasing (G+C) content: a simple formula for calculation of the Tm is
Tm = 4(G + C) + 2(A + T)oC.
Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s). One should aim at using an annealing temperature (Ta) about 5oC below the lowest Tm of their pair of primers to be used
1. Length should be between 18 and 30 nt, with optimal being 20-25 nt.
2.G-C content of 40-60% is desirable.
3.G-C should be evenly distributed ( i.e. no long run of G-C or A-T)
4.Try not to have run of A-T at the 3' and it is best to have G or C at 3' end.
5.The Tm should be between 55 C and 75 C. Warning: the old "4 degrees for each G-C, 2 degrees for each A-T" rule works poorly, especially for oligos shorter that 20 or longer than 25 nt.

Ordering process:
1.Choose a name for your primers
2. Define the sequence of your primer
3. Mark 3' and 5' ends of the primer
4.Specify the number of bases and required concentration of the primer
5. Order your primers online at www.kawsar.ir
6.The time of primer delivery is between 10-25 days.
7.If you have any question or problem contact us at oligo@kawsar.ir
8.Primers(for PCR) should be 17-28 bases in length
9.Base composition should be 50-60% (G+C)
10. Please make sure that you KEEP A COPY for your self (just in case you may need it.)

DNA	RNA
Crude(PCR-grade)	-
Salt-free	Salt-free
RP-HPLC	IE-HPLC
SDS-PAGE	SDS-PAGE

Scale	Price (Toman) / Base Pair
50 nmoles (OD 2)	400 / base
100 nmoles (OD 5)	600 / base
200 nmoles (OD 10)	1100 / base
1 µmoles (OD 25)	2500 / base